Single-cell Sequencing Reveals Brain/Spinal Cord Oligodendrocyte Precursor Heterogeneity and Requirement for mTOR in Cholesterol Biosynthesis and Myelin Maintenance

The goal of this study was to compare oligodendroglial heterogeneity specifically between brain and spinal cord and to determine the function of the mTOR in this heterogeneity. Oligodendroglia were isolated from developmentally stage matched brain (postnatal day 14) and spinal cord (postnatal day 10) based on expression of the O4 cell surface antigen (Miltenyl) and used for single cell RNA sequencing. Samples included control and mTOR conditional knockout (CNP-Cre; mTOR fl/fl) cells.本研究旨在特异性比较大脑与脊髓之间的少突胶质细胞异质性（oligodendroglial heterogeneity），并阐明哺乳动物雷帕霉素靶蛋白（mammalian target of rapamycin，mTOR）在该异质性中的功能。研究人员基于O4细胞表面抗原（Miltenyl）的表达，从发育阶段匹配的大脑（出生后第14天）与脊髓（出生后第10天）中分离少突胶质细胞，并将其用于单细胞RNA测序。样本包含对照组细胞与mTOR条件性敲除（CNP-Cre; mTOR fl/fl）细胞。