Preliminary report on the genetic structure of <i>Glyphoglossus molossus</i> (Anura: Microhylidae) from the Khorat Plateau, north-eastern Thailand

<i>Glyphoglossus molossus</i>, is one of the most economically important amphibians in the Khorat Plateau, north-eastern Thailand. It is categorised as a near-threatened species by IUCN due to over-exploitation. Here, we examined the genetic structure of <i>G. molossus</i> using partial mitochondrial cytochrome-b gene sequences from specimens collected at 11 localities. The sequence analysis revealed the presence of 15 haplotypes, 5 of which are shared by 2 or more populations, and 10 haplotypes that are confined to a single population. The relatively low haplotype (<i>h</i>) and nucleotide (<i>π</i>) diversities suggest that the Phu Phan Mountain Range is not high enough to act as an effective dispersal barrier between the Sakon Nakhon and Khorat Basins. However, AMOVA and phylogenetic analyses based on Maximum likelihood (ML) and Bayesian inference (BI) strongly supported the presence of two genetically divergent clades, Sakon Nakhon Basin and the northern part of the Khorat Basin (clade A) and the southern part of the Khorat Basin (clade B). These two lineages are separated by substantial geographical distance, which has putatively resulted in a reduction of gene flow. Further studies, with more extensive genetic sampling, especially from throughout the species range will aid in better interpreting our results.

钝头穴蛙（Glyphoglossus molossus）是泰国东北部呵叻高原（Khorat Plateau）最具经济重要性的两栖动物之一。因其遭受过度开发，被世界自然保护联盟（IUCN）列为近危物种。本研究利用采自11个采样点的样本的线粒体细胞色素b（cytochrome-b）基因部分序列，对钝头穴蛙的种群遗传结构展开分析。序列分析共检出15个单倍型，其中5个单倍型为2个及以上种群所共有，剩余10个单倍型仅局限于单个种群。较低的单倍型多样性（h）与核苷酸多样性（π）表明，普潘山脉（Phu Phan Mountain Range）不足以作为沙功那空盆地（Sakon Nakhon Basin）与呵叻盆地（Khorat Basins）之间的有效扩散屏障。然而，基于最大似然法（Maximum Likelihood, ML）与贝叶斯推断（Bayesian Inference, BI）的分子方差分析（Analysis of Molecular Variance, AMOVA）及系统发育分析，均强烈支持存在两个遗传分化显著的支系：沙功那空盆地与呵叻盆地北部构成支系A，呵叻盆地南部构成支系B。这两个支系间存在显著的地理阻隔，推测由此导致了基因交流的减少。后续需开展覆盖该物种全分布范围的更广泛遗传采样研究，以更好地阐释本研究结果。

搜集汇总

背景与挑战

背景概述

该数据集是关于泰国东北部Khorat Plateau近危物种Glyphoglossus molossus（一种经济重要的两栖动物）的遗传结构初步研究报告。研究基于线粒体细胞色素b基因序列分析，从11个地点采集样本，发现15个单倍型，并识别出两个遗传分化支系（Sakon Nakhon盆地和Khorat盆地北部支系A与Khorat盆地南部支系B），这些支系被地理距离分隔，可能导致基因流减少。