Dual roles for a tick protein disulfide isomerase during the life cycle of the Lyme disease agent [I. scapularis]

To determine whether the influences of Ixodes scapularis protein disulfide isomerase A6 (IsPDIA6) on Borrelia burgdorferi colonization is the result of physiological responses, we performed RNA-sequencing (RNA-seq) to compare the transcriptomes of control (GFP) and IsPDIA6-silenced ticks. We compared the transcriptome of GFP (control) and IsPDIA6-silenced ticks upon Borrelia burgdorferi infection.