Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.
Additional file 1. Plasmids for making parental HCT116 cells expressing OsTIR1 (Table S1), plasmids for the construction of CRISPR and donor plasmids for tagging (Table S2), primers used to generate t
Amplicon sequencing of edited alleles from the OpenCell project. In this project, a split-mNeonGreen tag sequence (90nt) is inserted via CRISPR-based HDR (homology-directed repair) into protein-coding
Interactions mediated by cell surface receptors initiate important instructive signaling cues but can be difficult to detect in biochemical assays because they are often highly transient and membrane-
