Hyb-Seq data for Inga

Current systematic hypotheses for the Neotropical tree genus Inga are very poorly resolved. We aim to use targeted enrichment and high-throughput sequencing methods to generate DNA sequence data for 1400 loci. Analyses indicate these data provide a robustly resolved phylogeny for the genus, even resolving population-level relationships within species (Nicholls et al., 2015). Libraries for each individual were made using Illuminas TruSeq Nano kit, with mean insert sizes of approximately 420 bp. Enrichment was performed using a MyBaits custom kit (manufactured by MYcroarray), with 4 - 12 samples pooled per capture reaction depending on library quality. Enriched pools were sequenced on an Illumina HiSeq using 150 base pair paired-end reads.