Cutting down steroid biosynthesis through SIRT6 induced H3K56ac deacetylation Fuels the osteogenic commitment of MSCs (ChIP-Seq)

To detect the mechanism underlying osteogenic commitment and proliferation rate change of HDPCs driven by MDL-800, we performed ChIP-seq analysis to detect the recruitment of genes on H3K9ac and H3K56ac in MDL-800 pre-treated HDPCs. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K9ac and H3K56ac for DMSO, MDL-800(agonist for SIRT6 deacetylase) pre-treated HDPCs.