Transcription profiling of mouse D10 testis from MILI+/- and MILI-/- animals

Spermatogenesis in MILI-KO mice was blocked completely at early prophase of the first meiosis during the zygotene and early pachytene stage. To analyze the molecular basis of MILI function, we examined the difference between gene expression for MILI-/- and MILI+/- testes. Experiment Overall Design: To exclude secondary effects, we prepared mRNA from day10 mice. Because the apoptotic cells in KO testis were observed at day11 after birth.MILI基因敲除（MILI-KO）小鼠的精子发生（Spermatogenesis）完全阻滞于第一次减数分裂早前期的合线期（zygotene）与早粗线期（early pachytene）。为解析MILI功能的分子基础，我们检测了MILI纯合敲除（MILI-/-）与杂合敲除（MILI+/-）小鼠睾丸的基因表达差异。实验整体设计：为排除次级效应，我们提取了出生后第10天小鼠的mRNA。这是由于敲除小鼠睾丸中的凋亡细胞（apoptotic cells）于出生后第11天即可被观测到。