COS7 tfx pNL4GFP and VPS4B(E235Q)

Tilt Series Date: 2012-03-15 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: Raptor Collaborators and Roles: VPS4B (E235Q) expression construct provided by W.Sundquist (Utah); pNLGFP construct provided by I. Chen (UCLA) Purification / Growth Conditions / Treatment: lipofectamine transfection Sample Preparation: manual blotting