Fig6. Interaction of AK154753/miR-345-3p/Bak and AK154753/miR-708-5p/Bim

(A-D) Luciferase reporter gene applied to explore the interaction of lncRNA, miRNA and mRNA. *P<0.05 vs. miR-NC, **P<0.01 vs. miR-NC. (E-F) flow cytometry to detect cell apoptosis after silencing of AK154753, as well as being transfected with miR-345-3p/miR-708-5p inhibitors in OGD/R model. (G-J) Western blotting detection of the expression of Bak/Bim after flow cytometry and Western blotting to detect cell apoptosis and apoptotic proteins after silencing of AK154753, as well as being transfected with miR-345-3p/miR-708-5p inhibitors in OGD/R model. **P<0.01 vs. control, ##P<0.01 vs. OGD/R+sh-NC, %%P<0.01 vs. OGD/R+sh-AK154753, &&P<0.01 vs. OGD/R+sh-AK154753, &P<0.05 vs. OGD/R+sh-AK154753.(n=3)