Blood miRNA as biomarkers of epilepsy (CTRL vs. SE)

We analyzed miRNA levels in blood samples using Affymetrix microarrays 4.1 and performed comparative analysis of samples. We performed 3 comparisons: i) control animals vs. Status epilepticus animals, ii) animals which developed first spontaneous seizure around 7 days post stimulation (EARLY), or later after 21days post stimulation (LATE), iii) animals that had high or low number of seizures as determined by seizures number (EARLY vs. LATE).本研究采用Affymetrix基因芯片4.1（Affymetrix microarrays 4.1）检测血液样本中的微小核糖核酸（microRNA，miRNA）表达水平，并对样本开展比较分析。共设置3组比较：① 对照组动物与癫痫持续状态（Status epilepticus）模型动物；② 刺激后约7天出现首次自发性癫痫发作的早期（EARLY）组动物，以及刺激后21天及以后出现首次发作的晚期（LATE）组动物；③ 依据癫痫发作次数划分为高发作组与低发作组，并在早期组与晚期组间开展该比较的动物。