Seawater carbonate chemistry and the response of brain and gill CRH systems of marine medaka to ocean acidification

In this study, marine medaka were exposed to CO2-acidified seawater (440 ppm, 1000 ppm, and 1800 ppm CO2) for 2 h, 4 h, 24 h, and 7 d, respectively. To elucidate the response of the CRH system to ocean acidification, the location and relative quantification of Crh in the brain and gills after acute (2 h, 4 h, and 24 h) and short-term (7 d) exposure to acidification, as well as the relative quantification of genes associated with the CRH system, were investigated.