TABLE 4 in Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures
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TABLE 4 Number of sequencing reads and Kraken2 k-mer fragments retained for analysis WGS sequencing data of pollen samples through processing steps of quality filtering and classification SampleTotal readsRetained after filteringClassified FragmentsUnclassified FragmentsLevel of classification Genus SpeciesMixture 12,737,8062,418,106706,013 (58.39%)503,040 (41.61%)680,193 (56.30%)669,890 (55.45%)Mixture 22,589,9702,163,054679,753 (62.85%)401,774 (37.15%)660,046 (61.04%)653,062 (60.38%)Mixture 31,832,2341,715,760639,808 (74.58%)218,072 (25.42%)624,267 (72.76%)603,821 (70.38%)Mixture 41,877,3601,795,644682,921 (76.06%)214,901 (23.94%)665,730 (74.19%)635,180 (70.77%)Mixture 51,583,9261,498,748744,876 (99.40%)4,498 (0.60%)731,490 (97.55%)563,733 (75.17%)Mixture 61,524,1281,381,686525,819 (76.11%)165,024 (23.89%)516,610 (74.78%)513,957 (74.38%)Mixture 71,841,2021,777,146581,375 (65.43%)307,198 (34.57%)560,525 (63.05%)550,258 (61.89%)Mixture 81,499,6921,421,518543,432 (76.46%)167,327 (23.54%)533,301 (75.04%)530,254 (74.61%)Mixture 91,294,5221,227,674458,933 (74.76%)154,904 (25.24%)450,772 (73.42%)448,198 (73.01%)Mixture 101,355,2421,208,520447,215 (74.01%)157,045 (25.99%)439,119 (72.64%)436,536 (72.21%)Mixture 112,018,3161,828,026673,577 (73.69%)240,436 (26.31%)661,246 (72.33%)657,267 (71.89%)Ambrosia artemisiifolia1,924,1761,877,500656,579 (69.94%)282,171 (30.06%)630,876 (67.23%)621,140 (66.16%)Artemisia tridentata1,770,4241,722,090554,707 (64.42%)306,338 (35.58%)534,603 (62.09%)524,307 (60.90%)Bassia scoparia1,790,2561,735,430839,951 (96.80%)27,764 (3.20%)832,989 (96.00%)831,720 (95.84%)Broussonetia papyrifera30145 (71.43%)2 (28.57%)5 (71.43%)5 (71.43%)Carya illinoinensis3,049,0022,687,2141,279,514 (95.23%)64,093 (4.77%)1253842 (93.29%)1,250,232 (92.97%)Poa pratensis1,817,9841,736,554640,084 (73.72%)228,193 (26.28%)628302 (72.35%)624,364 (71.89%)Populus deltoides1,613,0621,565,038773,325 (98.83%)9,194 (1.17%)758913 (96.94%)556,430 (71.07%)Populus tremuloides1,664,1881,613,334804,651 (99.75%)2,016 (0.25%)791915 (98.11%)624,746 (77.39%)Zea mays1,610,9261,438,576700,507 (97.39%)18,781 (2.61%)689128 (95.76%)688,837 (95.68%)Negative control 113,24210,096152 (45.65%)181 (54.35%)141 (42.34%)128 (38.43%)



