S1 File - Curcumin Mitigates the Intracellular Lipid Deposit Induced by Antipsychotics In Vitro

Fig A: Effect of different doses of curcumin on the intracellular DiI-LDL accumulation in cells treated with haloperidol. HepG2 cells were incubated with DiI-LDL (30 μg/ml of cholesterol) for 16 h in the absence (control) or the presence of haloperidol (10 μM). Then, cells were washed and serum-free medium containing the same antipsychotic without or with 5, 10, 30 or 40 μM curcumin was added and incubated for additional 2 h. Intracellular DiI-LDL was measured by flow cytometry. Results are mean ± SEM of three independent experiments performed in duplicate. M.I.F., median intensity of fluorescence; a.u.f., arbitrary units of fluorescence. Statistical comparisons shown are haloperidol versus control for each dose of curcumin (* PPPPPPP+ P (PDF)