Whole embryo depletion of Taf15 in gastrula and neurula stage Xenopus tropicalis embryos

We examined the role of Taf15 in in gastrula and neurula stage Xenopus tropicalis embryos. To determine the role of Taf15 in early Xenopus development, we used RNA-sequencing (RNA-seq) from single embryos depleted of M- and Z-Taf15, using reagents that targeted either all mRNA by inhibiting translation with Morpholino antisense and mismatch oligonucleotides (MOs) or only zygotic function using splice-blocking MOs or CRISPR-mediated mutagenesis.