Perturbation of gene expression for increased protein secretion in yeast.. Perturbation of gene expression for increased protein secretion in yeast.

Study to identify genes for which a decrease or an increase in gene expression resulted in increased secretion of α-amylase. Overall design: Perturbation of α-amylase secreting yeast cells using CRISPR/dCas9 plasmid activation or repression libraries, coupled with microfluidic screening for high secretion strains.