Identification of binding partners of the purified TOM complex from HEK293T cells

To investigate the interactome of the human translocase of the outer mitochondrial membrane (TOM) complex, we performed immunoaffinity purification of TOMM22-FLAG from HEK293T cells followed by LC-MS/MS analysis. This approach allowed us to identify proteins that co-purify with the TOM complex under native conditions. Our primary aim was to uncover human-specific interactors not previously described in yeast, with a particular focus on factors potentially involved in mitochondrial protein import regulation and quality control. The dataset provides a resource for understanding conserved and divergent aspects of TOM complex composition and function in human cells.