Here we describe the detection of influenza A virus sequences from 1918 obtained from three fomalin-fixed lung specimes preserved within the pathology collection of the medical museum of the Charite,
The PR8 and PR8mut viruses were produced by reverse genetics using the pHW191-PB2, pHW192-PB1, pHW193-PA, pHW194-HA, pHW195-NP, pHW196-NA, pHW197-M (wild type PR8) or pHW197-Mmut (PR8mut), and pHW198-
Influenza sequences from the NCBI (downloaded 2025-04-04). Metadata from AllNuclMetadata.csv.gz (https://ftp.ncbi.nlm.nih.gov/genomes/Viruses/AllNuclMetadata/) dropping certain unneeded columns (e.g.
