Carnoulès COWG metatranscriptome

Environmental RNA originates from the site of Carnoulès, Gard (France). It was extracted from the upper zone of a five centimeter deep white sediments collect sampled at the bottom of the Reigous creek, in a station called COWG, located 30 m downstream of the source of a small stream collecting ancient mine waste waters contaminated with (among others) zinc, lead and arsenic.The 2x100 paired-end libraries were sequenced on one full HiSeq2500 lane each.Combined with sequences from Carnoulès Metagenome Bioproject PRJNA62261, this study allowed the assembly of uncultivated Ferrovum CARN8 (PRJEB28721) and the determination of its metabolic activity in situ by metatranscriptomic analysis.