<b>Transcriptomic response of </b><b><i>Bacillus cereus</i></b><b> to potato-derived extracellular vesicles</b>

<i>Bacillus cereus</i> is a foodborne pathogen capable of forming persistent biofilms and producing enterotoxins, posing serious risks to food safety and public health. This dataset presents transcriptomic profiles of <i>B. cereus</i> ATCC 11778 following exposure to extracellular vesicles (EVs) derived from potato peels.RNA sequencing was performed to investigate the molecular mechanisms underlying the antimicrobial and antibiofilm activity of plant EVs. Analysis revealed that potato-derived EVs significantly suppressed critical bacterial pathways, including quorum sensing, two-component systems, ABC transporters, flagellar assembly, and oxidative phosphorylation. In contrast, EVs derived from milk promoted the expression of genes associated with growth and biosynthesis. These findings indicate that potato EVs act through a multi-target, cross-kingdom regulatory mechanism, disrupting bacterial virulence and survival strategies.The data support the potential of plant-derived EVs as sustainable, natural antimicrobial agents with applications in food preservation and biofilm control.蜡样芽孢杆菌（Bacillus cereus）是一种食源性致病菌，能够形成持久性生物被膜并产生肠毒素，对食品安全与公众健康构成严重威胁。本数据集收录了蜡样芽孢杆菌ATCC 11778暴露于源自马铃薯果皮的细胞外囊泡（extracellular vesicles，EVs）后的转录组谱。本研究通过RNA测序探究植物来源细胞外囊泡抗菌与抗生物被膜活性背后的分子机制。分析结果显示，马铃薯果皮来源的细胞外囊泡可显著抑制细菌的关键通路，包括群体感应（quorum sensing）、双组分调控系统（two-component systems）、ABC转运蛋白（ABC transporters）、鞭毛组装（flagellar assembly）以及氧化磷酸化（oxidative phosphorylation）。与之相反，牛乳来源的细胞外囊泡则会促进与生长及生物合成相关的基因表达。上述研究结果表明，马铃薯果皮来源的细胞外囊泡通过多靶点、跨界调控机制发挥作用，破坏细菌的毒力与生存策略。本数据集的数据证实，植物来源的细胞外囊泡具备成为可持续天然抗菌剂的潜力，可应用于食品保鲜与生物被膜防控领域。