Markers of nuclear and microsomal proteins in the plasma membrane and caveolae fractions.

Equal amounts of protein (10 μg) were subjected to SDS/PAGE, immunoblotting and densitometric scanning (Gel Doc 2000 imaging system, Bio-Rad). The results are expressed as the amount of the marker protein relative to the indicated subcellular fraction (set to 100%). The experiments shown are representative of 3 independent experiments. Abbreviations: plasma membrane (PM) fraction; caveolae membrane/lipid raft (CM) fraction.