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Supplemental_File_3_Primers.xlsx
Supplemental_File_3_Primers.xlsx
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NIAID Data Ecosystem
2026-03-11 收录
植物病原菌效应子
分子克隆
数据链接:
https://figshare.com/articles/dataset/Supplemental_File_3_Primers_xlsx/12463559
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资源简介:
Primer sequences for cloning and qRT-PCR of Bremia lactucae WY effectors.
应用场景:
创建时间:
2020-06-11
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Oligonucleotides used for the construction of the GFP fused truncated N proteins.
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Oligonucleotides used for the construction of the GFP fused truncated N proteins.
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Strains, plasmids and primers used in the study.
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Note: the enzyme sites are underlined and homologous regions are in italic.Strains, plasmids and primers used in the study.
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Primers for PCR of IFP35 promoter fragments and site-directed mutagenesis.
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Primers for PCR of IFP35 promoter fragments and site-directed mutagenesis.
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Additional file 8 of Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci
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Additional file 8. PCR condition details for all eight projects. Table highlighting the PCR condition details for all eight projects.
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Primers used to amplify recombinant wild- type and attenuated spEX from Staphylococcus pseudintermedius and pUC19- spEX- M plasmid.
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NotI and BamHI restriction sites are bold and underlined, nucleotide sequences belonging to SpEX and SpEX-M are show in italics, the primer binding sites are in brackets and sequences outside SpEX and
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