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Minimize off-target edits for base editors
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创建时间：
2023-03-10
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Additional file 6: of Correction to: An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes)
Table S3. Potential off-target sites of 7 candidates of bait sequence that selected in the first screening and previously reported bait sequences. Potential off-target sites are defined as genomic seq
NIAID Data Ecosystem150
Ectopic expression of RAD52 and dn53BP1 improves homology-directed repair during CRISPR/Cas9 genome editing. Homo sapiens
Gene disruption by CRISPR/Cas9 is highly efficient and relies on the error-prone non-homologous end-joining (NHEJ) pathway. Conversely, precise gene editing requires homology-directed repair (HDR), wh
NIAID Data Ecosystem80
Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
Even the latest generation of base editor (BE3) causes unwanted indels and non-C-to-T substitutions, compromising the fidelity of base editing outcome. Here we report a enhanced base editing system. T
NIAID Data Ecosystem30
Engineering a miniaturized SaCas9 adenine base editor with reduced RNA off-targets and increased on-target DNA editing efficiencies. Protein domain-altered adenine base editors
We compared the editing profile of circularly permuted and domain inlaid Cas9 base editors, and found that on-target editing can be largely maintained following their intradomain insertion, but that s
NIAID Data Ecosystem80
On targeting specificity of Cas12b nucleases
On targeting specificity of Cas12b nucleases
NIAID Data Ecosystem50
