美洲杨树的促雌基因FERR和抑雌基因FERR-R及其应用

利用美洲黑杨的全同胞家系群体和自然群体，对美洲黑杨的性别决定位点进行精细定位。对定位区间进行序列解析，发现区间内的FERR-R基因是由FERR基因的片段复制产生的，且通过RdDM机制沉默FERR基因。结合拟南芥的遗传转化实验，验证了FERR基因是美洲黑杨的促雌基因，为后续的无絮杨树的创制提供了重要的靶基因。相关内容已获国家发明专利授权。We conducted fine-mapping of the sex-determining loci in *Populus deltoides* using its full-sib family population and natural population. Sequence analysis of the mapped interval showed that the FERR-R gene within the interval was derived from segmental duplication of the FERR gene, and the FERR gene is silenced via the RdDM mechanism. Combined with genetic transformation experiments in *Arabidopsis thaliana*, we verified that the FERR gene functions as a female-promoting gene in *Populus deltoides*, which provides a critical target gene for the subsequent creation of fluff-free poplar varieties. This research has been granted a national invention patent in China.

搜集汇总

背景与挑战

背景概述

该数据集涉及美洲杨树的性别决定机制研究，通过精细定位和序列分析，发现促雌基因FERR和由它复制产生的抑雌基因FERR-R，后者通过RdDM机制沉默前者。实验验证了FERR的促雌功能，为培育无絮杨树提供了关键靶基因。