Spatiotemporal transcriptional dynamics of the cycling mouse oviduct

Female fertility in mammals requires the iterative remodeling of the entire adult female reproductive tract across the menstrual/estrous cycle. Here we examine global transcriptional dynamics of the mouse oviduct along the anteroposterior axis and across the estrous cycle. Though we observed robust patterns of differential oviduct gene expression along the anteroposterior axis, we found surprisingly few changes in gene expression across the estrous cycle, in marked contrast to other mammals. We speculate that this is an evolutionarily derived state that may reflect the extremely rapid five-day mouse estrous cycle. Mouse oviducts from 7-9 week-old estrous-cycle-staged Swiss Webster female mice were linearized and dissected in duplicate for each of the four estrous stages across both anterior and posterior regions. RNA was extracted from the oviduct Anterior: stage Proestrus n=2, Estrus n=2, Metestrus n=2, Diestrus n=2. RNA was extracted from the oviduct Posterior: stage Proestrus n=2, Estrus n=2, Metestrus n=2, Diestrus n=2.哺乳动物雌性生育功能的维持，依赖于成年雌性全生殖道在月经/动情周期中发生的迭代重塑。本研究针对小鼠输卵管（oviduct）沿前后轴以及动情周期的全局转录动态展开分析。尽管观察到小鼠输卵管沿前后轴存在显著的差异基因表达模式，但令人意外的是，动情周期中输卵管的基因表达变化极少，这与其他哺乳动物的情况形成鲜明对比。我们推测，这是一种演化衍生的状态，可能反映了小鼠仅5天的极快动情周期特征。 实验采用7-9周龄、经动情周期分期的瑞士韦伯斯特（Swiss Webster）雌性小鼠的输卵管，将其纵向拉直后解剖，针对四个动情阶段的前后两段区域，每个分组均设置两份生物学重复。 输卵管前段的RNA提取分组如下：发情前期（Proestrus）n=2、发情期（Estrus）n=2、发情后期（Metestrus）n=2、间情期（Diestrus）n=2。 输卵管后段的RNA提取分组如下：发情前期（Proestrus）n=2、发情期（Estrus）n=2、发情后期（Metestrus）n=2、间情期（Diestrus）n=2。