Investigation of B cell markers in secondary lymphoid organs

This dataset was generated as part of a multi-omics study aimed at investigating human B cell development. The study included bulk and single cell epigenetic methodologies coupled with single cell transcriptomics and proteomics. It demonstrated the emergence of epigenetic differences in B cell subsets at early stages of development that discriminate between divergent developmental paths. Epigenetic features established at early stages are retained in distinct mature cell subpopulations, where they are coupled with transcription of accessible genomic regions. Identified features are retained in secondary lymphoid organs. Data included in this submission were generated using Imaging Mass Cytometry on FFPE tissue sections from human tonsil, appendix and spleen. The antibody panel used included features of B cell subsets, and additional immune and epithelial cell markers. Transcriptomic features identified in the study were also included for validation in secondary lymphoid organs.