Peripheral human neutrophils were isolated as above and pretreated with 50 μg/mL of purified human THP, sialic acid (500 ng/mL, catalog no. A0812, Sigma Aldrich), or mock-treated, and incubated at 37°
Representative flow-cytometry diagrams of cells treated with 0 μM(A), 100 μM(A), 200 μM(A), and 400 μM(A) UR for a 48-h 4-d period after starvation for 24 h. The a,b means columns with different lette
LysoTracker FitC data in text files after processing from flow cytometry files. Samples were quantified in an Attune Flow Cytometer with the absorption and emission set at 504 and 511 nm.
