Coverage of BGT-positive area by presynaptic markers in healthy and dilute muscles.

Peronaeus muscles of healthy and dilute mice were explanted at P19. Muscles were fixed, sliced and co-stained with postsynaptic BGT-AF647 and antibodies against presynaptic synaptophysin or neurofilament M. Upon confocal microscopy and preparation of maximum z-projections, co-localisation between post- and presynaptic markers was determined. Data show the average fraction of the BGT-AF647-positive area in individual NMJs, which was covered by synaptophysin or neurofilament M immunofluorescence signals in percent ±s.e.m. (n-values, number of analysed microscopic fields). The number of analysed NMJs was >40 from at least two different muscles for each condition.