The accuracy of sequencing single DNA molecules with nanopores is continually improving, but de novo genome sequencing and assembly using only nanopore data remain challenging. Here we describe PoreSe
These files contain the nanopore long reads produced by R9.4 MinION, basecalled and demultiplexed by Albacore v2.1.3. Half of each strain's sample underwent end repair, and half did not - these wer
Zipped fast5 files from Nanopore sequencing of wild-type Escherichia coli cells grown at 45C, corresponding to biological replicate 2 as described in NCBI GEO accession GSM8513540.
Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at