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<i>C. elegans </i>bacteria colonization data set. Wild type (N2) vs. pmk-1 knockout (KU25) worms colonized with GFP-labeled E. coli OP50 bacteria

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DataCite Commons2025-01-13 更新2025-04-16 收录
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This is a <i>C. elegans </i>dataset used to test and validate the Systematic Imaging of <i>Caenorhabditis </i>Killing Organisms (SICKO) imaging and analysis pipeline. The dataset consists of raw TIF image files containing fluorescent images of individual <i>C. elegans</i> cultured in custom single-worm culture environments (DOI: 10.3791/64682). Wild type (strain N2) or pmk-1 knockout (strain KU25) <i>C. elegans</i> were challenged with GFP-labeled <i>E. coli </i>OP50 bacteria, washed to remove non-adherent bacteria, and transferred to single-worm culture environment wells with unlabeled bacteria. Each worm was imaged daily to quantify fluorescent intestinal bacterial colonies.<br><br><i>For inquiries regarding the contents of this dataset, please contact the Corresponding Author listed in the README.txt file. Administrative inquiries (e.g., removal requests, trouble downloading, etc.) can be directed to data-management@arizona.edu</i>

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2024-05-06
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