ChIP-chip analyses of H3K9 methylation in rrp6 deletion and rrp6 alp13 double mutant S.pombe cells.. Schizosaccharomyces pombe

ChIP-chip analyses of H3K9 methylation in rrp6 deletion (exosome mutant) and in rrp6 alp13 double mutant S.pombe cells. Keywords: ChIP-chip Overall design: Chromatin immunoprecipitated DNA recovered with antibody against methylated H3K9 from asynchronous growing fission yeast cells and whole cell extracts DNA were amplified by random priming and respectively labeled with Cy5 and Cy3. Labeled DNA samples were mixed and hybridized onto a custom Agilent 44K in situ 60mer DNA microarray that tiles the entire Schizosaccharomyces pombe genome every 300 bp alternately on both strands. Data were processed using Agilent scanner and Feature Extraction software.