RNA-Seq analysis of ada2?, nrg1? and cir1? and KN99a wildtype cells in capsule inducing and non-inducing conditions

To contextualize the cryptococcal Spt-Ada-Gcn5 Acetyltransferase (SAGA) member Ada2 in the capsule regulatory network, RNA-Seq was performed on wildtype, ada2? and two other transcription factors, nrg1? and cir1?, which like ada2? are also defective for capsule formation, virulence and filamentation. Overall design: Mutant and wildtype cells were grown in capsule inducing and non-inducing conditions for 90 minutes and transcriptional profiling was performed by RNA-Seq analysis.