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Differentially expressed cytokine genes in AT-MSCs from three different donors, cultured to low or high density, as determined by microarray analysis.

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Viable second-passage AT-MSCs plated at 200 cells/cm2 (CC1 MSCs) or 5,000 cells/cm2 (CC2 MSCs) were incubated for 7 days by which time they reached ∼50% or ∼90% confluence, respectively. After harvesting, total mRNA was isolated from pooled samples of MSCs from three donors and used in the microarray analysis. Microarray data were filtered by applying two criteria for significance, P<0.05 and FC>2 between culture conditions.

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2014-01-06
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