Spatial localization is a key determinant of cellular fate and behavior, but spatial RNA assays traditionally rely on staining for a limited number of RNA species. In contrast, single-cell RNA-seq all
Contains the raw images and MatLab scripts to show localization of recruited (Ser5P) and elongating (Ser2P) RNA polymerase II to the zebrafish microRNA miR-430 cluster. Embryos were fixed in the oblon
One of the greatest strengths of “-omics” technologies is their ability to capture a molecular snapshot of multiple cellular processes simultaneously. Transcriptomics, proteomics, and metabolomics hav
We have sequenced the polysome-associated translating mRNAs from stage-matched wild-type and eif3ha morphant embryos at ~24 hpf stage to identify transcripts translationally regulated by eIF3ha. As a