RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes during human erythroipoiesis
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Purpose: The goals of this study are to determine the differentially expreseed non-coding RNAs during human erythropoiesis Methods:gene expression profiling with cells on days 4,8,11,14 during erythroid differentiation, with two replicated using Illumina Genome Analyzer IIx. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: ncRNAs were differentially expressed during erythroid differentiation mRNA profiles of day4,8,11,14 erythroid cells were generated by deep sequencing, in duplicate.
创建时间：
2019-02-21
