Data supporting <b>The Proximal Centriole-Like Structure Maintains Nucleus-Centriole Architecture in Sperm</b>

These data include all the images used for this publication. Each zip file contains all the data used to produce a single figure in the manuscript.Publication AbstractProper connection between the sperm head and tail is critical for sperm motility and fertilization. Head-tail linkage is mediated by the Head-Tail Coupling Apparatus (HTCA), which secures the axoneme (tail) to the nucleus (head). However, the molecular architecture of the HTCA is poorly understood. Here, we use <i>Drosophila</i> to investigate formation and remodeling of the HTCA throughout spermiogenesis by visualizing key components of this complex. Using structured illumination microscopy, we demonstrate that key HTCA proteins Spag4 and Yuri form a ‘Centriole Cap’ that surrounds the centriole (or Basal Body) as it invaginates into the surface of the nucleus. As development progresses, the centriole is laterally displaced to the side of the nucleus while the HTCA expands under the nucleus, forming what we term the ‘Nuclear Shelf.’ We next show that the proximal centriole-like (PCL) structure is positioned under the Nuclear Shelf, functioning as a critical stabilizer of centriole-nuclear attachment. Together, our data indicate that the HTCA is a complex, multi-point attachment site that simultaneously engages the PCL, the centriole, and the nucleus to ensure proper head-tail connection during late-stage spermiogenesis.

本数据集包含本出版物所用的全部图像。每个压缩包均包含手稿中单幅插图制作所需的全部数据。
出版物摘要：
精子头尾部的正确连接对于精子活力与受精过程至关重要。头尾连接由头尾连接装置（Head-Tail Coupling Apparatus, HTCA）介导，该装置将轴丝（尾部）固定于细胞核（头部）之上。然而，目前学界对HTCA的分子结构尚缺乏深入认知。本研究以果蝇（Drosophila）为模型，通过可视化该复合物的关键组分，探究整个精子发生过程中HTCA的形成与重塑过程。借助结构照明显微镜，本研究证实HTCA的关键蛋白Spag4与Yuri可形成“中心粒帽”，在中心粒（或称基体）内陷进入细胞核表面时包裹该结构。随着发育进程推进，中心粒发生侧向位移至细胞核一侧，与此同时HTCA在细胞核下方扩展，形成我们命名为“细胞核支架”的结构。后续研究发现，近端中心粒样（proximal centriole-like, PCL）结构定位于细胞核支架下方，可作为中心粒与细胞核附着过程的关键稳定因子。综上，本研究数据表明HTCA是一种复杂的多位点附着结构，可同时与PCL、中心粒及细胞核结合，以确保精子发生晚期实现头尾部的正确连接。

搜集汇总

背景与挑战

背景概述

该数据集支持一项关于精子中近端中心粒样结构维持核-中心粒架构的研究，包含所有用于出版物的图像文件，每个zip文件对应一个图表。数据集聚焦于精子头部和尾部连接机制，涉及HTCA和PCL结构的分子架构分析，使用果蝇模型和结构化照明显微镜技术，关键词涵盖Sperm、HTCA、PCL等，适用于细胞发育研究领域。