We describe a strategy, which we term hypothesis-driven multiple-stage mass spectrometry (HMS-MS), for the sensitive detection and identification of phosphopeptides derived from enzymatic digests of p
Identification of in vitro PKA phosphorylation sites in human titin fragment (amino acids 9689-9988, NCBI:NP_001254479.2) fused to TEV cleavage site and HALOtag (amino acids 3-296, NCBI:AQS79242.1) .
