In vitro expansion affects the response of human bone marrow stromal cells to irradiation

Cell passage in vitro affected the irradiation response of BMSCs via molecular mechanisms that mediated differences in apoptosis, the cell cycle, senescence and the cytokines secretion. Overall design: BMSCs were passaged in vitro and irradiated at passage 6 (P6) and passage 10 (P10). The gene expression profile were analysed for the P6, P10, and non-irradiated (0h) BMSCs at different post-irradiation time points (2h and 8h).