ASSISTED REPRODUCTIVE TECHNOLOGIES ALTER DNA METHYLATION PROFILES IN BLOODSPOTS OF NEWBORN INFANTS . Homo sapiens

The Illumina Infinium HumanMethylation 450k Beadchip was employed to study the impact of in-vitro fertilization on DNA methylation in peripheral blood of infants. Using a total of 137 Michigan newborns born through IVF using fresh embryo transfer, IVF using cryopreserved embryo transfer, Intrauterine Insemination, or unassisted conception, DNA from archived Guthrie cards was assayed for methylation profiles. Overall design: A study in differential methylation, DNA from 137 archived bloodspots were bisulphite converted and assayed using the Infinium HumanMethylation 450k Beadchip