miRNA expression analysis of Salmonella Typhimurium infected cells

To have a global picture of the miRNAs regulated upon Salmonella infection, we assessed small RNA changes, by RNA-sequencing, of HeLa cells infected with Salmonella Typhimurium compared with mock-treated cells . In addtion to the total population, we evaluated miRNA expression in the fraction of HeLa cells with internalized bacteria (Salmonella-positive), as well as in bystander cells, separated by fluorescence activated cell sorting (FACS) Overall design: miRNome profile of Salmonella infected cells (total), mock-treated cells (mock), sorted cells with internalized bacteria (Salmonella positive) and sorted cells without internalized bacteria (bystander) cells were generated by RNA-sequencing