CONDITIONALLY LETHAL RICE ENVIRONMENT MUTATION GENE OSESL1, PROTEIN ENCODED THEREBY, AND APPLICATION THEREOF
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Provided are a conditionally lethal rice environment mutation gene osesl1, a protein encoded thereby, and an application thereof, resolving the issue in which existing methods fail to effectively prevent the effects of abnormal climate on the fertility of a photo-thermo-sensitive male sterile line. The nucleotide sequence of the gene osesl1 is as set forth in the sequence listing SEQ ID NO: 1, and the amino acid sequence of the protein encoded thereby is as in set forth in SEQ ID NO: 2. Following heading of osesl1 mutated rice, the lethal embryonic phenotype occurs 12 days after pollination, manifesting as blackening at a junction of the embryo and the endosperm. The embryo is normal at an average temperature lower than 22??C, and dies at an average temperature higher than 28??C. When the temperature is between 22-28??C, the embryo dies during a long-daylight regime longer than 13 hours, and is normal during a short-daylight regime shorter than 13 hours. Also provided is an application of the gene osesl1 in controlling the development of rice embryos.
本发明公开了一种条件致死型水稻环境突变基因osesl1、其编码的蛋白及其应用,解决了现有技术无法有效抵御异常气候对光温敏雄性不育系育性产生负面影响的技术难题。该基因osesl1的核苷酸序列如序列表SEQ ID NO:1所示,其编码的蛋白的氨基酸序列如SEQ ID NO:2所示。在osesl1基因突变水稻中,授粉后12天即可观察到胚胎致死表型,具体表现为胚与胚乳连接处发黑。当平均温度低于22℃时,胚胎发育正常;当平均温度高于28℃时,胚胎死亡。当温度处于22℃-28℃区间时,若处于每日光照时长超过13小时的长日照条件下,胚胎会死亡;若处于每日光照时长不足13小时的短日照条件下,胚胎发育正常。本发明还提供了osesl1基因在调控水稻胚胎发育中的应用。




