DNA Repair Profiling of HGSC Organoids

In this study, 34 ovarian tumor organoids generated from 23 patients underwent paired DNA damage repair defect functional analyses and whole exome sequencing analysis to compare genetic mutational status to DNA repair functional capacity. 22 of the patients had high grade serous cancer and 1 of the patients had low grade serous cancer. A normal control, fresh parent tumor, and cells from the organoid culture generated from the parent tumor underwent whole exome sequencing analysis and the data from the sequencing analysis is provided here.]]> All patients with ovarian cancer regardless of age or ethnicity had the option to participate. Men were excluded from this work as ovarian cancer only effects women.]]> A cohort of 23 patient samples was collected between April 2017 and April 2018 including blood or tissue normal controls and tumor tissue. Organoid cultures were generated from the tumor tissue. A normal control, fresh frozen parent tumor tissue, and a cell pellet from the organoid culture underwent whole exome sequencing for each patient, and some patients had more than one parent tumor site and organoid culture. A total of 34 organoid/parent tumor pairs along with matched normal controls underwent analysis.]]>