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RNA-mediated activity-based protein profiling reveals the substrates of a human dihydrouridine synthase. RNA-mediated activity-based protein profiling reveals the substrates of a human dihydrouridine synthase

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NIAID Data Ecosystem2026-03-12 收录
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We adapted the iCLIP method to map DUS3L-dependent DHU sites in a transcriptome-wide manner by treating the cells with 5-FUrd. Overall design: Flp-In T-Rex 293 cells expressing 3xFlag-DUS3L were treated with 5-FUrd, DUS3L-RNA were extracted and subject for Iclip library preparation.

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2021-05-31
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