Expression analysis of TCRgd+LAP+ versus TCRgd+LAP- cells

We used RNA-Seq to identify differential gene expression in LAP+ vs. LAP- Gamma-delta T-cells. We report a signature of 407 genes that were enriched in TCRgd+LAP+ vs. TCRgd+LAP- cells with p<0.05. Among the up-regulated genes, we found increased expression of genes related to antigen presentation, including MHC class II molecules (H2-Aa, H2-Ab1, H2-Eb1 and H2-Eb2), CD40 and CD86 RNA-Seq of TCRgd+LAP+ versus TCRgd+LAP- cells harvested from mouse spleen and Peyer's patches; began with 20 mice for two independent experiments (10 mice each); samples were pooled and for each experiment resulted in one LAP- and one LAP+ sample for RNA-SEQ, or four samples in total; The two LAP+ were combined, resulting in three samples in total for RNA-Seq.本研究采用RNA测序（RNA-Seq）鉴定LAP阳性（LAP+）与LAP阴性（LAP-）γδ T细胞（Gamma-delta T-cells）中的差异基因表达。我们报道了一组共407个基因的特征标记，这些基因在TCRγδ阳性且LAP阳性（TCRgd+LAP+）细胞中相较于TCRγδ阳性且LAP阴性（TCRgd+LAP-）细胞显著富集，且P值均小于0.05。在上调基因中，我们发现与抗原呈递相关的基因表达上调，包括主要组织相容性复合体II类（MHC class II）分子（H2-Aa、H2-Ab1、H2-Eb1及H2-Eb2）、CD40与CD86。本实验针对从小鼠脾脏和派尔集合淋巴结（Peyer's patches）中分离获取的TCRgd+LAP+与TCRgd+LAP-细胞开展RNA-Seq：两次独立实验各使用10只小鼠，合计20只；每个实验分别制备1份LAP-样本与1份LAP+样本用于RNA测序，两轮实验总计得到4份样本；随后将两份LAP+样本合并，最终得到3份用于RNA-Seq的样本。