Single-cell transcriptome profiling of primary tumors and paired organoids of pancreatic cancer

Pancreatic cancer (PC) is the most lethal common gastrointestinal malignancy with an overall 5-year survival rate of 11%. Recently, a variety of patient-derived models, including patient-derived xenograft (PDX) and cancer organoid models, have paved a way for identifying common responders to chemotherapy. However, the utility of PC organoid for recapitulating hallmarks of corresponding primary tumor at transcriptome level remains to be investigated. We conducted single-cell RNA sequencing (scRNA-seq) for three paired primary tumors and organoids using the 10x Genomics platform to identify common and different phenotypes between them. All fresh resection specimens and cultured organoids were enzymatically digested to generate single-cell suspensions for scRNA-seq. ----------------------------------------- Authors state "The corresponding raw data will be submitted to SRA."