Changes in gene profiles during the long term culture of human induced pluripotent stem cell-derived cardiomyocytes

To investigate the changes in gene profiles during the long-term culture process of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes, we conducted microarray analysis on hiPSC(253G4 line)- derived cardiomyocytes at three time points: 20 days, 35 days, and 50 days after the initiation of differentiation induction. hiPSC(253G4 line)- derived cardiomyocytes were cultured in low-glucose medium (MEMα) plus 5% fetal bovine serum (FBS) and samples were collected at three time points: 20 days, 35 days, and 50 days after the initiation of differentiation induction. Subsequently we performed microarray analysis on each samples.