Stroma extracts were isolated from 2-week-old WT plants and incubated with either BSF-specific antibodies or with the pre-immune serum. IgGs were captured with SiMAG-Protein G beads (Chemicell) and re
To discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional N-terminally Tandem Affinity Purification (TAP) tagged ALG-1 protein We purified TAP::ALG-1 complexes f
Assessing the influence of RNA structural and sequence variations on SNRPA1 binding preference in vitro Overall design: Recombinant SNRPA1 protein was incubated with SSE RNA and SSE RNA with structura
Flag-RIP-seq was performed in NAT10-Flag overexpressing iSLK-KSHV cells. NAT10-Flag and its control RFP-Flag was overexpressed in iSLK-KSHV cells, the Flag-RIP-seq was performed.