The Androgen Receptor Directly Regulates CD44 Expression in Androgen Sensitive Bladder Cancer [gene expression]

To study androgen receptor transcriptomic activation in the presence of androgen in bladder cancer cell lines. A uniquely derived AR expressing clone of the bladder cancer cell line UMUC3 was derived and validated prior to microarray experiments. Microarrays were used to determine the overall expression changes in repsonse to treatment with synthetic androgen. Evaluation of genes associated with androgen receptor stimulation were used for focused analysis when combined with ChIP-seq data. Cell were treated with vehicle control (ethanol) or synthetic androgen (R1881) with 3 biological replicates per condition