Univariate analyses of variance of the percentage cover of biofilm on panels submerged in different sites in all sampling times.

n = 4. The whole analyses is shown as well as the a priori contrast (i.e. the analyses where the distances to be compared were chosen a priori). ns = not significant; * = p<0.05; ** = p<0.01. 600 m and 1400 m = sites 600 and 1400 m away from the effluent, respectively. N and S are sites in different sides of the bay (see Methods). Factor 1: Distance (Di), fixed, with 2 levels; Factor 2: Sites (Si), random, nested in Distance with 4 levels; Factor 3: Time (Ti), random, with 2 levels. Data were arc-sin transformed.