Localization of Arp2/3 complex at actin filament branching points. Xenopus keratocytes and fibroblasts were treated with CD (0.2 μM for 30 min or 0.5 μM for 10 min), extracted in the presence of phall
To avoid spectral interference with common fluorophores in multicolor fluorescence microscopy, a fluid-phase tracer with excitation and emission in the violet end of the visible spectrum is desirable.
The rapid development of highly multiplexed microscopy systems has enabled the study of cells embedded within their native tissue. The rich spatial data provided by these techniques have yielded excit