Kinetic properties <i>in vitro</i> of the enzymes involved in Ins biosynthesis in <i>O</i>. <i>mossambicus</i>.

Initial rates with different substrate concentrations were measured In vitro, and kinetic properties (KM, Vmax and kcat) were determined by non-linear regression analysis using GraphPad 5 software. Values for three separate experiments are shown (units: KM, mM ± SE; Vmax, nmol sec-1 ± SE; kcat, sec-1 ± SE; kcat/KM, sec-1·mM-1 ± SE). Eight substrate concentrations were used for determination of kinetic properties. Asterisks represent parameters with significant differences (t-test, P < 0.05) for IMPase 1 assayed with different substrates (Ins-1P vs Ins-3P). Kinetic properties in vitro of the enzymes involved in Ins biosynthesis in O. mossambicus.